GSM5696554: Zfp335WT scRNA-seq; Mus musculus; RNA-Seq - SRA

SRX13181823: GSM5696554: Zfp335WT scRNA-seq; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 468.6M spots, 55.8G bases, 16.8Gb downloads

External Id: GSM5696554_r1

Submitted by: Zhuang/ Shinohara, Immunology, Duke University

Study: Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-B-selection pre-T cells [scRNA-seq]

PRJNA782189 • SRP347038 • All experiments • All runs

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The transcription factor Zfp335 is esstential for the survival of post-B-selection DN4 thymocytes. We utilized 10x Genomics v3 platform to profile the transcriptomes of Overall design: DN4 thymocytes were FACS-sorted (Live Lin- CD4- CD8- CD44- CD25-) from Zfp335-fl/fl or Zfp335-fl/fl E8III-cre positive 6 week old mice. Each sample was a pool of one male and one female mouse. Following sorting, scRNA-seq libraries were prepared using the 10x Chromium system with v3 chemistry targeting 7,000 cells per sample.

Sample: Zfp335WT scRNA-seq

SAMN23341772 • SRS11111207 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM5696554

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: DN4 thymocytes were isolated by FACS sorting. Single-cell suspensions were loaded on the 10x Genomics Chromium controller, and mRNA converted to cDNA lirbaries. Single-cell libraries were sequenced on a Novaseq 6000 150PE S-Prime flowcell. Libraries were seuqenced in single index mode with the following read lengths: 28x8x91 10x Genomics 3' kit v3

Runs: 1 run, 468.6M spots, 55.8G bases, 16.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR16991466	468,609,041	55.8G	16.8Gb	2022-09-13

ID:: 18013865

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